The development of nutrient media for the cultivation of recombinant Escherichia coli strains

The study of the qualitative and quantitative composition of peptides and free amino acids in commercial casein hydrolysates, produced in Russia, has provided grounds for the choice of the nutrient base in the medium for the cultivation of E. coli recombinant strains. In the newly developed medium s...

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Bibliographic Details
Published inŽurnal mikrobiologii, ėpidemiologii i immunobiologii no. 5; p. 6
Main Authors Santsevich, N I, Kadomtseva, V M, Aminov, R I, Denisova, S V, Smirnova, G A, Mel'nikova, V A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.1994
Subjects
Bacterial Proteins - biosynthesis
Cellulase - analysis
Cellulase - metabolism
Culture Media - analysis
Culture Media - metabolism
Escherichia coli - growth & development
Protein Hydrolysates - analysis
Protein Hydrolysates - metabolism
Recombinant Proteins - biosynthesis
Recombination, Genetic
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Summary:The study of the qualitative and quantitative composition of peptides and free amino acids in commercial casein hydrolysates, produced in Russia, has provided grounds for the choice of the nutrient base in the medium for the cultivation of E. coli recombinant strains. In the newly developed medium specific endoglucanase activity is 1.5- to 1.7-fold greater than in Luria-Bertani broth ("Difco" Laboratories, USA).
ISSN:0372-9311