The outer membranes of Vibrio cholerae as a potential component in a chemical vaccine

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii Vol. Suppl 2; p. 86
• Main Authors: Markov, E Iu, Urbanovich, L Ia, Golubinskiĭ, E P, Chernov, A B, Karetnikova, E S, Ivanova, T A, Pakulev, N A
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.03.1995
• Subjects: Animals; Antibodies, Bacterial - blood; Bacterial Outer Membrane Proteins - immunology; Bacterial Outer Membrane Proteins - toxicity; Cholera - prevention & control; Cholera Vaccines - immunology; Cholera Vaccines - toxicity; Dose-Response Relationship, Immunologic; Drug Evaluation, Preclinical; Immunization; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Rabbits; Time Factors; Vaccines, Synthetic - immunology; Vaccines, Synthetic - toxicity; Vibrio cholerae - immunology
• ISSN: 0372-9311

The results of the study of the preparation of V. cholerae eltor membrane, obtained by the lysis and inactivation of microbial cells with urea and the subsequent differential centrifugation and nuclease treatment. As revealed in this study, the outer membrane preparation, when introduced parenterally and orally to mice, induced pronounced immunity to experimental cholera infection and the production of vibriocidal antibodies in high titers. The treatment of V. cholerae eltor membranes with trypsin led to further increase of the immunogenic potency of the preparation. The protective action of V. cholerae eltor outer membranes considerably exceeded the protective effect of currently used whole-cell eltor vaccine. This opens prospects for using the above-mentioned preparation for the improvement of chemical vaccine as a component ensuring the formation of antibacterial immunity.