Detection of antibodies against ovum antigens in Schistosoma mansoni bilharziasis

Fifty-five sera from bilharziasis patients from West Indies and thirty-five control sera (from normal subjects and patients with hydatidosis and fascioliasis) were examined by ELISA for antibodies reacting with S. mansoni soluble egg antigen MSA1 . MSA1 antigen was prepared according to Pelley and c...

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Bibliographic Details
Published inBulletin de la Societe de pathologie exotique et de ses filiales Vol. 76; no. 5; p. 514
Main Authors Tribouley, J, Tribouley-Duret, J, Lacassie, I, Appriou, M, Sauneron, M F
Format Journal Article
LanguageFrench
Published France 01.11.1983
Subjects
Animals
Antibodies - isolation & purification
Antigen-Antibody Reactions
Antigens - immunology
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoelectrophoresis
Immunoenzyme Techniques
Mice
Ovum - immunology
Schistosoma mansoni - immunology
Schistosomiasis - immunology
Time Factors
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Summary:Fifty-five sera from bilharziasis patients from West Indies and thirty-five control sera (from normal subjects and patients with hydatidosis and fascioliasis) were examined by ELISA for antibodies reacting with S. mansoni soluble egg antigen MSA1 . MSA1 antigen was prepared according to Pelley and coupled to isothiocyanate substituted plastic discs. Sensitivity and specificity of reaction were good. Moreover, in experimental infection in mice, sera of mice cured with praziquantel became negative. The sera were also examined by a whole egg antigen in counter immunoelectrophoresis. The results of the two different techniques correspond well although ELISA using purified antigen prove to be more specific and sensitive.