Cationic fluorinated micelles for cell labeling and 19 F-MR imaging

• Published in: Scientific reports Vol. 14; no. 1; p. 22613
• Main Authors: Jirát-Ziółkowska, Natalia, Panakkal, Vyshakh Manayath, Jiráková, Klára, Havlíček, Dominik, Sedláček, Ondřej, Jirák, Daniel
• Format: Journal Article
• Language: English
• Published: England 30.09.2024
• Subjects: Animals; Cations - chemistry; Cell Survival - drug effects; Contrast Media - chemistry; Fluorine - chemistry; Fluorine-19 Magnetic Resonance Imaging - methods; Halogenation; Humans; Magnetic Resonance Imaging - methods; Mice; Micelles; Phantoms, Imaging; Staining and Labeling - methods; 19F magnetic resonance spectroscopy; Fluorinated micelles; Cell labeling; 19F magnetic resonance imaging
• ISSN: 2045-2322
• DOI: 10.1038/s41598-024-73511-8

Magnetic resonance imaging (MRI) relies on appropriate contrast agents, especially for visualizing transplanted cells within host tissue. In recent years, compounds containing fluorine-19 have gained significant attention as MRI probe, particularly in dual H/ F-MR imaging. However, various factors affecting probe sensitivity, such as fluorine content and the equivalency of fluorine atoms, must be considered. In this study, we synthesized fluorinated micelles with adjustable surface positive charge density and investigated their physicochemical properties and MRI efficacy in phantoms and labeled cells. While the micelles exhibited clear signals in F-MR spectra and imaging, the concentrations required for MRI visualization of labeled cells were relatively high, adversely affecting cell viability. Despite their favourable physicochemical properties, achieving higher labeling rates without compromising cell viability during labeling remains a challenge for potential in vivo applications.