Halotolerant Endophytic Bacteria Priestia flexa 7BS3110 with Hg 2+ Tolerance Isolated from Avicennia germinans in a Caribbean Mangrove from Colombia

• Published in: Microorganisms (Basel) Vol. 12; no. 9
• Main Authors: Soto-Varela, Zamira E, Orozco-Sánchez, Christian J, Bolívar-Anillo, Hernando José, Martínez, José M, Rodríguez, Nuria, Consuegra-Padilla, Natalia, Robledo-Meza, Alfredo, Amils, Ricardo
• Format: Journal Article
• Language: English
• Published: Switzerland 07.09.2024
• Subjects: mercury biosorption; exopolysaccharides; heavy metal; endophytes
• ISSN: 2076-2607; 2076-2607

The mangrove ecosystems of the Department of Atlántico (Colombian Caribbean) are seriously threatened by problems of hypersalinization and contamination, especially by heavy metals from the Magdalena River. The mangrove plants have developed various mechanisms to adapt to these stressful conditions, as well as the associated microbial populations that favor their growth. In the present work, the tolerance and detoxification capacity to heavy metals, especially to mercury, of a halotolerant endophytic bacterium isolated from the species located in the Balboa Swamp in the Department of Atlántico was characterized. Diverse microorganisms were isolated from superficially sterilized leaves. Tolerance to NaCl was evaluated for each of the obtained isolates, and the most resistant was selected to assess its tolerance to Pb , Cu , Hg , Cr , Co , Ni , Zn , and Cd , many of which have been detected in high concentrations in the area of study. According to the ANI and AAI percentages, the most halotolerant strain was identified as , named 7BS3110, which was able to tolerate up to 12.5% ( / ) NaCl and presented a minimum inhibitory concentrations (MICs) of 0.25 mM for Hg, 10 mM for Pb, and 15 mM for Cr . The annotation of the 7BS3110 genome revealed the presence of protein sequences associated with exopolysaccharide (EPS) production, thiol biosynthesis, specific proteins for chrome efflux, non-specific proteins for lead efflux, and processes associated with sulfur and iron homeostasis. Scanning electron microscopy (SEM) analysis showed morphological cellular changes and the transmission electron microscopy (TEM) showed an electrodense extracellular layer when exposed to 0.25 mM Hg . Due to the high tolerance of 7BS3110 to Hg and NaCl, its ability to grow when exposed to both stressors was tested, and it was able to thrive in the presence of 5% ( / ) NaCl and 0.25 mM of Hg . In addition, it was able to remove 98% of Hg from the medium when exposed to a concentration of 14 mg/L of this metalloid. 7BS3110 has the potential to bioremediate Hg halophilic contaminated ecosystems.