Increase of secondary metabolites in sweet basil (Ocimum basilicum L.) leaves by exposure to N 2 O 5 with plasma technology

Exposure to N O generated by plasma technology activates immunity in Arabidopsis through tryptophan metabolites. However, little is known about the effects of N O exposure on other plant species. Sweet basil synthesizes many valuable secondary metabolites in its leaves. Therefore, metabolomic analys...

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Bibliographic Details
Published inScientific reports Vol. 14; no. 1; p. 12759
Main Authors Tateishi, Rie, Ogawa-Kishida, Natsumi, Fujii, Nobuharu, Nagata, Yuji, Ohtsubo, Yoshiyuki, Sasaki, Shota, Takashima, Keisuke, Kaneko, Toshiro, Higashitani, Atsushi
Format Journal Article
LanguageEnglish
Published England 04.06.2024
Subjects
Eugenol - analogs & derivatives
Eugenol - metabolism
Flavonoids - metabolism
Gene Expression Regulation, Plant
Metabolomics - methods
Ocimum basilicum - genetics
Ocimum basilicum - metabolism
Oxylipins - metabolism
Plant Leaves - drug effects
Plant Leaves - genetics
Plant Leaves - metabolism
Secondary Metabolism - drug effects
Metabolomics
Plasma technology
Secondary metabolites
Dinitrogen pentoxide
Transcriptional upregulation
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Summary:Exposure to N O generated by plasma technology activates immunity in Arabidopsis through tryptophan metabolites. However, little is known about the effects of N O exposure on other plant species. Sweet basil synthesizes many valuable secondary metabolites in its leaves. Therefore, metabolomic analyses were performed at three different exposure levels [9.7 (Ex1), 19.4 (Ex2) and 29.1 (Ex3) μmol] to assess the effects of N O on basil leaves. As a result, cinnamaldehyde and phenolic acids increased with increasing doses. Certain flavonoids, columbianetin, and caryophyllene oxide increased with lower Ex1 exposure, cineole and methyl eugenol increased with moderate Ex2 exposure and L-glutathione GSH also increased with higher Ex3 exposure. Furthermore, gene expression analysis by quantitative RT-PCR showed that certain genes involved in the syntheses of secondary metabolites and jasmonic acid were significantly up-regulated early after N O exposure. These results suggest that N O exposure increases several valuable secondary metabolites in sweet basil leaves via plant defense responses in a controllable system.
ISSN:2045-2322