Enhancement of vitamin B 6 production driven by omics analysis combined with fermentation optimization

• Published in: Microbial cell factories Vol. 23; no. 1; p. 137
• Main Authors: Tian, Zhizhong, Liu, Linxia, Wu, Lijuan, Yang, Zixuan, Zhang, Yahui, Du, Liping, Zhang, Dawei
• Format: Journal Article
• Language: English
• Published: England 15.05.2024
• Subjects: Amino Acids - metabolism; Carbon - metabolism; Citric Acid Cycle; Escherichia coli - genetics; Escherichia coli - metabolism; Fermentation; Metabolic Engineering - methods; Metabolic Networks and Pathways; Metabolome; Metabolomics; Nitrogen - metabolism; Pyridoxine - metabolism; Transcriptome; Vitamin B 6 - biosynthesis; Vitamin B 6 - metabolism; Cellular processes; Fermentation optimization; Omics analysis; Vitamin B6
• ISSN: 1475-2859

Microbial engineering aims to enhance the ability of bacteria to produce valuable products, including vitamin B for various applications. Numerous microorganisms naturally produce vitamin B , yet the metabolic pathways involved are rigorously controlled. This regulation by the accumulation of vitamin B poses a challenge in constructing an efficient cell factory. In this study, we conducted transcriptome and metabolome analyses to investigate the effects of the accumulation of pyridoxine, which is the major commercial form of vitamin B , on cellular processes in Escherichia coli. Our omics analysis revealed associations between pyridoxine and amino acids, as well as the tricarboxylic acid (TCA) cycle. Based on these findings, we identified potential targets for fermentation optimization, including succinate, amino acids, and the carbon-to-nitrogen (C/N) ratio. Through targeted modifications, we achieved pyridoxine titers of approximately 514 mg/L in shake flasks and 1.95 g/L in fed-batch fermentation. Our results provide insights into pyridoxine biosynthesis within the cellular metabolic network for the first time. Our comprehensive analysis revealed that the fermentation process resulted in a remarkable final yield of 1.95 g/L pyridoxine, the highest reported yield to date. This work lays a foundation for the green industrial production of vitamin B in the future.