Identification and expression profile analysis of rice CRF gene family

• Published in: Sheng wu gong cheng xue bao = Chinese journal of biotechnology Vol. 40; no. 4; p. 1157
• Main Authors: Song, Yongsen, Yu, Yang, Tan, Chuhe, Meng, Qi, Gou, Yurui, Duan, Xiangbo
• Format: Journal Article
• Language: Chinese
• Published: China 25.04.2024
• Subjects: Abscisic Acid - metabolism; Abscisic Acid - pharmacology; Cytokinins - metabolism; Gene Expression Profiling; Gene Expression Regulation, Plant; Multigene Family; Oryza - genetics; Oryza - metabolism; Phylogeny; Plant Growth Regulators - metabolism; Plant Growth Regulators - pharmacology; Plant Proteins - genetics; Plant Proteins - metabolism; Stress, Physiological - genetics; Transcription Factors - genetics; Transcription Factors - metabolism; cytokinin response factors; gene family analysis; rice; expression profile
• ISSN: 1872-2075

Cytokinin response factors (CRFs), as unique transcription factors in plants, play crucial roles in regulating development, phytohormone signaling pathway, and stress responses. In this study, we identified nine genes from the rice genome by conducting a BLAST analysis using the protein sequences of twelve AtCRFs. These genes are located on seven different rice chromosomes. We conducted a comprehensive analysis of the conserved domains, physicochemical properties, secondary structures, and phylogenetic relationships of rice CRF proteins using various online tools and local software. Additionally, we analyzed the exon-intron structures and -acting elements of , and found an abundance of elements relevant to phytohormone response and stress response on the promoters of rice genes. Spatial-temporal expression pattern analysis revealed that four of the were barely expressed in all tested samples, while the other five were highly expressed in the leaf, panicle, or seed of rice. Microarray data showed that genes ar