Neurobehavioral dysfunction in a mouse model of Down syndrome: upregulation of cystathionine β-synthase, H 2 S overproduction, altered protein persulfidation, synaptic dysfunction, endoplasmic reticulum stress, and autophagy

• Published in: GeroScience Vol. 46; no. 5; p. 4275
• Main Authors: Panagaki, Theodora, Janickova, Lucia, Petrovic, Dunja, Zuhra, Karim, Ditrói, Tamás, Jurányi, Eszter P, Bremer, Olivier, Ascenção, Kelly, Philipp, Thilo M, Nagy, Péter, Filipovic, Milos R, Szabo, Csaba
• Format: Journal Article
• Language: English
• Published: Switzerland 01.10.2024
• Subjects: Aminooxyacetic Acid - pharmacology; Animals; Autophagy; Behavior, Animal; Brain - metabolism; Cystathionine beta-Synthase - genetics; Cystathionine beta-Synthase - metabolism; Disease Models, Animal; Down Syndrome - genetics; Down Syndrome - metabolism; Down Syndrome - physiopathology; Endoplasmic Reticulum Stress - physiology; Female; Hydrogen Sulfide - metabolism; Male; Mice; Synapses - metabolism; Up-Regulation; Brain; Persulfidation; Gliosis; Astrocytes; Gasotransmitters; Cognition; Metabolism
• ISSN: 2509-2723

Down syndrome (DS) is a genetic condition where the person is born with an extra chromosome 21. DS is associated with accelerated aging; people with DS are prone to age-related neurological conditions including an early-onset Alzheimer's disease. Using the Dp(17)3Yey/ + mice, which overexpresses a portion of mouse chromosome 17, which encodes for the transsulfuration enzyme cystathionine β-synthase (CBS), we investigated the functional role of the CBS/hydrogen sulfide (H S) pathway in the pathogenesis of neurobehavioral dysfunction in DS. The data demonstrate that CBS is higher in the brain of the DS mice than in the brain of wild-type mice, with primary localization in astrocytes. DS mice exhibited impaired recognition memory and spatial learning, loss of synaptosomal function, endoplasmic reticulum stress, and autophagy. Treatment of mice with aminooxyacetate, a prototypical CBS inhibitor, improved neurobehavioral function, reduced the degree of reactive gliosis in the DS brain, increased the ability of the synaptosomes to generate ATP, and reduced endoplasmic reticulum stress. H S levels in the brain of DS mice were higher than in wild-type mice, but, unexpectedly, protein persulfidation was decreased. Many of the above alterations were more pronounced in the female DS mice. There was a significant dysregulation of metabolism in the brain of DS mice, which affected amino acid, carbohydrate, lipid, endocannabinoid, and nucleotide metabolites; some of these alterations were reversed by treatment of the mice with the CBS inhibitor. Thus, the CBS/H S pathway contributes to the pathogenesis of neurological dysfunction in DS in the current animal model.