High- or Low-Yielding F 2 Progeny of Wheat Is Result of Specific TaCKX Gene Coexpression Patterns in Association with Grain Yield in Paternal Parent

• Published in: International journal of molecular sciences Vol. 25; no. 6
• Main Authors: Szala, Karolina, Dmochowska-Boguta, Marta, Bocian, Joanna, Orczyk, Wacław, Nadolska-Orczyk, Anna
• Format: Journal Article
• Language: English
• Published: Switzerland 21.03.2024
• Subjects: Genotype; Oxidoreductases - metabolism; Phenotype; Plant Breeding; Seedlings; Triticum - genetics; Triticum - metabolism; cytokinins; expression pattern; paternal inheritance; NAC2; wheat yield; TaCKX; breeding; epigenetic imprinting
• ISSN: 1422-0067

Members of the gene family (GFM) encode oxidase/dehydrogenase cytokinin degrading enzymes (CKX), which play an important role in the homeostasis of phytohormones, affecting wheat development and productivity. Therefore, the objective of this investigation was to test how the expression patterns of the yield-related genes and ( ) measured in 7 days after pollination (DAP) spikes and the seedling roots of parents are inherited to apply this knowledge in the breeding process. The expression patterns of these genes were compared between parents and their F progeny in crosses of one mother with different paterns of awnless cultivars and reciprocal crosses of awned and awnless lines. We showed that most of the genes tested in the 7 DAP spikes and seedling roots of the F progeny showed paternal expression patterns in crosses of awnless cultivars as well as reciprocal crosses of awned and awnless lines. Consequently, the values of grain yield in the F progeny were similar to the pater; however, the values of seedling root mass were similar to the mother or both parents. The correlation analysis of GFMs and in spikes and spikes per seedling roots reveals that the genes correlate with each other specifically with the pater and the F progeny or the mother and the F progeny, which shape phenotypic traits. The numbers of spikes and semi-empty spikes are mainly correlated with the specific coexpression of the and genes expressed in spikes or spikes per roots of the pater and F progeny. Variable regression analysis of grain yield and root mass with GFMs and expressed in the tested tissues of five crosses revealed a significant dependency of these parameters on the mother and F and/or the pater and F progeny. We showed that the inheritance of yield-related traits depends on the specific cooperative expression of some GFMs, in some crosses coupled with , and is strongly dependent on the genotypes used for the crosses. Indications for parental selection in the breeding of high-yielding lines are discussed.