Adaptive mechanism of the marine bacterium Pseudomonas sihuiensis-BFB-6S towards pCO 2 variation: Insights into synthesis of extracellular polymeric substances and physiochemical modulation

Marine bacteria can adapt to various extreme environments by the production of extracellular polymeric substances (EPS). Throughout this investigation, impact of variable pCO levels on the metabolic activity and physiochemical modulation in EPS matrix of marine bacterium Pseudomonas sihuiensis - BFB...

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Published inInternational journal of biological macromolecules Vol. 261; no. Pt 2; p. 129860
Main Authors Chakraborty, Subham, Paidi, Murali Krishna, Dhinakarasamy, Inbakandan, Sivakumar, Manikandan, Clements, Clarita, Thirumurugan, Naren Kumar, Sivakumar, Lakshminarayanan
Format Journal Article
LanguageEnglish
Published Netherlands 01.03.2024
Subjects
Extracellular Polymeric Substance Matrix
Hydrogen-Ion Concentration
Pseudomonas
Seawater
Spectroscopy, Fourier Transform Infrared
Extracellular polymeric substances
Spectroscopic annotation
Biofilm
Physiological adaptation
Marine bacteria
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Summary:Marine bacteria can adapt to various extreme environments by the production of extracellular polymeric substances (EPS). Throughout this investigation, impact of variable pCO levels on the metabolic activity and physiochemical modulation in EPS matrix of marine bacterium Pseudomonas sihuiensis - BFB-6S was evaluated using a fluorescence microscope, excitation-emission matrix (EEM), 2D-Fourier transform infrared correlation spectroscopy (2D-ATR-FTIR-COS), FT-NMR and TGA-DSC. From the results at higher pCO levels, there was a substantial reduction in EPS production by 58-62.8 % (DW). In addition to the biochemical composition of EPS, reduction in carbohydrates (8.7-47.6 %), protein (7.1-91.5 %), and lipids (16.9-68.6 %) content were observed at higher pCO levels. Functional discrepancies of fluorophores (tyrosine and tryptophan-like) in EPS, speckled differently in response to variable pCO . The 2D-ATR-FTIR-COS analysis revealed functional amides (CN, CC, CO bending, -NH bending in amines) of EPS were preferentially altered, which led to the domination of polysaccharides relevant functional groups at higher pCO . H NMR analysis of EPS confirmed the absence of chemical signals from H-C-COOH of proteins, α, β anomeric protons, and acetyl group relevant region at higher pCO levels. These findings can contribute new insights into the influence of pCO on the adaptation of marine microbes in future ocean acidification scenarios.
ISSN:1879-0003