Determination of acetochlor by UPLC-MS 3 in cells and its application to a cellular pharmacokinetic study

The aim of this work was to develop a fast, simple, and reliable UPLC-MS method for the sensitive detection of acetochlor in biological samples. In MS mode, the ion transition m/z 270.1 → 224.1→148.1 was chosen for quantification with butachlor as the internal standard. In the UPLC system, separatio...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 688; p. 115476
Main Authors Lu, Di, Zheng, Xinyue, Xue, Hongyu, You, Jiansong, Yin, Lei, Shi, Meiyun
Format Journal Article
LanguageEnglish
Published United States 01.05.2024
Subjects
UPLC-MS
Acetochlor
Cellular pharmacokinetics
Herbicide
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Summary:The aim of this work was to develop a fast, simple, and reliable UPLC-MS method for the sensitive detection of acetochlor in biological samples. In MS mode, the ion transition m/z 270.1 → 224.1→148.1 was chosen for quantification with butachlor as the internal standard. In the UPLC system, separation was performed on a UPLC column (2.1 × 50 mm ID, 1.7 μm) with 0.1 % FA in water and acetonitrile as mobile phases. After simple protein precipitation via acetonitrile, the method was well validated with good linearity (0.5-20 ng/mL, r > 0.995), accuracy (-3.70 %-2.98 %), and precision (<15 %). The selectivity and sensitivity were improved obviously in MS mode than that in MRM mode. The developed UPLC-MS method was successfully applied to the cellular pharmacokinetics study of acetochlor in MCF-7 cells.
ISSN:1096-0309