Anti-Inflammatory Effects of Alphitolic Acid Isolated from Agrimonia coreana Nakai Extracts Are Mediated via the Inhibition of I CRAC Activity in T Cells

• Published in: International journal of molecular sciences Vol. 24; no. 24
• Main Authors: Park, Su Jin, Lee, Jin Seok, Nam, Yu Ran, Lee, Ji Min, Ki, Dae-Won, Yun, Bong-Sik, Choi, Seong Woo, Van, Nhung Thi Hong, Nam, Joo Hyun, Kim, Hyun Jong, Kim, Woo Kyung
• Format: Journal Article
• Language: English
• Published: Switzerland 09.12.2023
• Subjects: Agrimonia pilosa; Agrimonia coreana; CD4+ T cell; stromal interaction molecule 1 (STIM1); alphitolic acid; CRACs (Ca2+ release activated Ca2+ channels); anti-inflammation; ORAI
• ISSN: 1422-0067

Ledeb., an important medicinal herb in traditional East Asian medicine, is primarily used to treat abdominal pain, dysentery, and hemostasis. There are ten other reported species of plants, including Nakai-a naturally growing species in South Korea-and Linn. Although recent studies have isolated numerous active constituents and investigated their effects, the medicinal utility of this herb is not yet fully explored. Through patch-clamp recording, a previous study reported that plant extracts inhibit the function of Ca release-activated Ca channels (CRACs). Herein, we aimed to identify and isolate the main compounds in responsible for CRAC inhibition while assessing the anti-inflammatory effects mediated by this inhibition. We demonstrated for the first time that alphitolic acid isolated from has a dose-dependent inhibitory effect on CRAC activity and, thus, an inhibitory effect on intracellular calcium increase. Furthermore, analysis of human CD4 T cell proliferation via the carboxyfluorescein diacetate succinimidyl ester method revealed that alphitolic acid inhibited T cell proliferation in a concentration-dependent manner. Our findings provide a theoretical basis for the potential therapeutic use of alphitolic acid in the treatment of inflammatory diseases.