Cold-induced suspension and resetting of Ca 2+ and transcriptional rhythms in the suprachiasmatic nucleus neurons

• Published in: iScience Vol. 26; no. 12; p. 108390
• Main Authors: Enoki, Ryosuke, Kon, Naohiro, Shimizu, Kimiko, Kobayashi, Kenta, Hiro, Sota, Chang, Ching-Pu, Nakane, Tatsuto, Ishii, Hirokazu, Sakamoto, Joe, Yamaguchi, Yoshifumi, Nemoto, Tomomi
• Format: Journal Article
• Language: English
• Published: United States 15.12.2023
• Subjects: Molecular neuroscience; Neuroscience; Biological sciences; Cellular neuroscience
• ISSN: 2589-0042
• DOI: 10.1016/j.isci.2023.108390

Does the circadian clock keep running under such hypothermic states as daily torpor and hibernation? This fundamental question has been a research subject for decades but has remained unsettled. We addressed this subject by monitoring the circadian rhythm of clock gene transcription and intracellular Ca in the neurons of the suprachiasmatic nucleus (SCN), master circadian clock, under a cold environment. We discovered that the transcriptional and Ca rhythms are maintained at 22°C-28°C, but suspended at 15°C, accompanied by a large Ca increase. Rewarming instantly resets the Ca rhythms, while transcriptional rhythms reach a stable phase after the transient state and recover their phase relationship with the Ca rhythm. We conclude that SCN neurons remain functional under moderate hypothermia but stop ticking in deep hypothermia and that the rhythms reset after rewarming. These data also indicate that stable Ca oscillation precedes clock gene transcriptional rhythms in SCN neurons.