Influence of family with sequence similarity 134, member B-mediated reticulophagy on lipopolysaccharide-induced apoptosis of mouse dendritic cells

To investigate the influence of family with sequence similarity 134, member B (FAM134B)-mediated reticulophagy on lipopolysaccharide (LPS)-induced apoptosis of mouse dendritic cells (DCs), so as to provide a basis for improving the immune suppression of sepsis caused by wound infection and other fac...

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Bibliographic Details
Published inZhonghua shao shang yu chuang mian xiu fu za zhi Vol. 39; no. 9; p. 857
Main Authors Duan, Y, Yao, R Q, Zheng, L Y, Dong, N, Wu, Y, Yao, Y M, Dai, X G
Format Journal Article
LanguageChinese
Published China 20.09.2023
Subjects
Animals
Apoptosis
Autophagy
bcl-2-Associated X Protein
Caspase 3
Dendritic Cells - pathology
Lipopolysaccharides - pharmacology
Mice
RNA, Small Interfering
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Summary:To investigate the influence of family with sequence similarity 134, member B (FAM134B)-mediated reticulophagy on lipopolysaccharide (LPS)-induced apoptosis of mouse dendritic cells (DCs), so as to provide a basis for improving the immune suppression of sepsis caused by wound infection and other factors. The experimental research methods were used. The DC line DC2.4 of the 3 to 10 passage in the logarithmic growth stage was collected for experiments. DCs were divided into LPS stimulation 0 h (no stimulation) group, LPS stimulation 6 h group, LPS stimulation 12 h group, LPS stimulation 24 h group, and LPS stimulation 72 h group, which were cultured with 1 μg/mL LPS (the same concentration below) for the corresponding time. The protein expressions of FAM134B, microtubule-associated protein 1 light chain 3B (LC3B), and transporter protein SEC61B were determined by Western blotting, and the ratio of LC3B-Ⅱ/LC3B-Ⅰ was calculated ( =3). DCs were divided into phosphate buffer solution (PBS) group and LPS group for c
ISSN:2097-1109