Expression changes of Na V channel subunits correlate with developmental maturation of electrophysiological characteristics of rat cerebellar Purkinje neurons

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 43; no. 7; p. 1102
• Main Authors: Fu, M, Ji, X, Zhong, L, Wu, Q, Li, H, Wang, N
• Format: Journal Article
• Language: Chinese
• Published: China 20.07.2023
• Subjects: Animals; Brain; Neurons; Purkinje Cells - physiology; Rats; Rats, Sprague-Dawley; Sodium - metabolism; correlation analysis; spontaneous electrical activity; Purkinje neuron development; immunohistochemistry; voltage gated sodium channel
• ISSN: 1673-4254

To investigate the variations in the expression of voltage-gated sodium (Na ) channel subunits during development of rat cerebellar Purkinje neurons and their correlation with maturation of electrophysiological characteristics of the neurons. We observed the changes in the expression levels of Na 1.1, 1.2, 1.3 and 1.6 during the development of Purkinje neurons using immunohistochemistry in neonatal (5-7 days after birth), juvenile (12-14 days), adolescent (21-24 days), and adult (42-60 days) SD rats. Using whole-cell patch-clamp technique, we recorded the spontaneous electrical activity of the neurons in ex vivo brain slices of rats of different ages to analyze the changes of electrophysiological characteristics of these neurons during development. The expression of Na subunits in rat cerebellar Purkinje neurons showed significant variations during development. Na 1.1 subunit was highly expressed throughout the developmental stages and increased progressively with age ( < 0.05). Na 1.2 expression was not dete