Effect of glycosaminoglycans with different degrees of sulfation on chondrogenesis

This study aims to investigate the effects and mechanisms of chondroitin sulfate (CS), dermatan sulfate (DS), and heparin (HEP) on chondrogenesis of murine chondrogenic cell line (ATDC5) cells and the maintenance of murine articular cartilage . ATDC5 and articular cartilage tissue explant were cultu...

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Published inHua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology Vol. 41; no. 4; p. 395
Main Authors Zheng, Wen, Cai, Ming-Xiang, Peng, Huizhen, Liu, Minyi, Liu, Xiangning
Format Journal Article
LanguageEnglish
Published China 01.08.2023
Subjects
Animals
Cartilage
Cell Differentiation
Cells, Cultured
Chondrocytes - metabolism
Chondrogenesis - physiology
Glycosaminoglycans - metabolism
Glycosaminoglycans - pharmacology
Mice
degree of sulfation
chondrogenesis
cartilage repair
sulfated glycosaminoglycan
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Summary:This study aims to investigate the effects and mechanisms of chondroitin sulfate (CS), dermatan sulfate (DS), and heparin (HEP) on chondrogenesis of murine chondrogenic cell line (ATDC5) cells and the maintenance of murine articular cartilage . ATDC5 and articular cartilage tissue explant were cultured in the medium containing different sulfated glycosaminoglycans. Cell proliferation, differentiation, cartilage formation, and mechanism were observed using cell proliferation assay, Alcian blue staining, real-time quantitative polymerase chain reaction (RT-qPCR), and Western blot, respectively. Results showed that HEP and DS primarily activated the bone morphogenetic protein (BMP) signal pathway, while CS primarily activated the protein kinase B (AKT) signal pathway, further promoted ATDC5 cell proliferation and matrix production, and increased Sox9, Col2a1, and Aggrecan expression. This study investigated the differences and mechanisms of different sulfated glycosaminoglycans in chondrogenesis and cartilage homeostasis maintenance. HEP promotes cartilage formation and maintains the normal state of cartilage tissue , while CS plays a more effective role in the regeneration of damaged cartilage tissue.
ISSN:2618-0456