Transcriptomic analysis reveals the functions of H 2 S as a gasotransmitter independently of Cys in Arabidopsis
Numerous studies have revealed the gasotransmitter functions of hydrogen sulfide (H S) in various biological processes. However, the involvement of H S in sulfur metabolism and/or Cys synthesis makes its role as a signaling molecule ambiguous. The generation of endogenous H S in plants is closely re...
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Published in | Frontiers in plant science Vol. 14; p. 1184991 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
2023
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Subjects | |
Online Access | Get full text |
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Summary: | Numerous studies have revealed the gasotransmitter functions of hydrogen sulfide (H
S) in various biological processes. However, the involvement of H
S in sulfur metabolism and/or Cys synthesis makes its role as a signaling molecule ambiguous. The generation of endogenous H
S in plants is closely related to the metabolism of Cys, which play roles in a variety of signaling pathway occurring in various cellular processes. Here, we found that exogenous H
S fumigation and Cys treatment modulated the production rate and content of endogenous H
S and Cys to various degrees. Furthermore, we provided comprehensive transcriptomic analysis to support the gasotransmitter role of H
S besides as a substrate for Cys synthesis. Comparison of the differentially expressed genes (DEGs) between H
S and Cys treated seedlings indicated that H
S fumigation and Cys treatment caused different influences on gene profiles during seedlings development. A total of 261 genes were identified to respond to H
S fumigation, among which 72 genes were co-regulated by Cys treatment. GO and KEGG enrichment analysis of the 189 genes, H
S but not Cys regulated DEGs, indicated that these genes mainly involved in plant hormone signal transduction, plant-pathogen interaction, phenylpropanoid biosynthesis, and MAPK signaling pathway. Most of these genes encoded proteins having DNA binding and transcription factor activities that play roles in a variety of plant developmental and environmental responses. Many stress-responsive genes and some Ca
signal associated genes were also included. Consequently, H
S regulated gene expression through its role as a gasotransmitter, rather than just as a substrate for Cys biogenesis, and these 189 genes were far more likely to function in H
S signal transduction independently of Cys. Our data will provide insights for revealing and enriching H
S signaling networks. |
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ISSN: | 1664-462X 1664-462X |