Comparative evaluation of methods for detecting HBsAg in sera

The results of the analysis of 1209 serum samples, made with a view to detecting those containing HBsAg, are presented. This analysis was made by the radioimmunoassay (RIA) on a polyethylene film, by the standard RIA technique with the use of a diagnostic kit obtained from Abbott Laboratories (USA)...

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Bibliographic Details
Published inŽurnal mikrobiologii, ėpidemiologii i immunobiologii no. 5; p. 12
Main Authors Semenova, N P, Prokudina, E N, Bocharova, N G, Shalunova, N V, Zhdanov, V M
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1986
Subjects
Autoradiography
Evaluation Studies as Topic
Hemagglutination Tests - methods
Hepatitis B Surface Antigens - analysis
Humans
Radioimmunoassay - instrumentation
Radioimmunoassay - methods
Reagent Kits, Diagnostic
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Summary:The results of the analysis of 1209 serum samples, made with a view to detecting those containing HBsAg, are presented. This analysis was made by the radioimmunoassay (RIA) on a polyethylene film, by the standard RIA technique with the use of a diagnostic kit obtained from Abbott Laboratories (USA) and by the passive hemagglutination (PHA) test. The RIA film technique was found to have the sensitivity of about 2 ng/ml HBsAg, which is similar to the sensitivity of the kit from Abbott Laboratories and exceeds the sensitivity of the PHA test approximately 50-fold. The percentage of detected HBsAg-positive sera, yielded by analysis with the use of the RIA film technique and the standard RIA technique, is the same. The RIA technique make it possible to detect more positive sera than the PHA test by about 2.5%.
ISSN:0372-9311