Click chemistry-based novel albumin nanoparticles for anticancer treatment via H 2 O 2 generation

Glucose oxidase (GOD) exerts anticancer effects by producing hydrogen peroxide (H O ). However, the use of GOD is limited by its short half-life and low stability. Systemic H O production following systemic absorption of GOD can also cause serious toxicity. GOD-conjugated bovine serum albumin nanopa...

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Bibliographic Details
Published inColloids and surfaces, B, Biointerfaces Vol. 226; p. 113335
Main Author Lee, Changkyu
Format Journal Article
LanguageEnglish
Published Netherlands 01.06.2023
Subjects
Azides
Cell Line, Tumor
Click Chemistry
Drug Carriers
Hydrogen Peroxide
Nanoparticles
Particle Size
Serum Albumin, Bovine
Glucose oxidase
Nanoparticle
Albumin
Click chemistry
Tumor therapy
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Summary:Glucose oxidase (GOD) exerts anticancer effects by producing hydrogen peroxide (H O ). However, the use of GOD is limited by its short half-life and low stability. Systemic H O production following systemic absorption of GOD can also cause serious toxicity. GOD-conjugated bovine serum albumin nanoparticles (GOD-BSA NPs) may be useful for overcoming these limitations. Here, bioorthogonal copper-free click chemistry was employed to develop GOD-BSA NPs that are non-toxic and biodegradable and can effectively and rapidly conjugate proteins. These NPs retained their activity, unlike conventional albumin NPs. NPs using dibenzyl cyclooctyne (DBCO)-modified albumin, azide-modified albumin, and azide-modified GOD were fabricated in 10 min. After intratumoral administration, GOD-BSA NPs remained in the tumor for a longer period and displayed better anticancer activity than the effects of GOD alone. GOD-BSA NPs were approximately 240 nm in size and inhibited tumor growth to 40 mm , whereas tumors treated with phosphate-buffered saline or albumin NPs had sizes of 1673 and 1578 mm , respectively. GOD-BSA NPs prepared using click chemistry may be useful as a drug delivery system for protein enzymes.
ISSN:1873-4367