Phosphate-driven H 2 O 2 decomposition on DNA-bound bio-inspired activated carbon-based sensing platform for biological and food samples

Hydrogen peroxide (H O ) is one of the most important reactive oxygen species (ROS). Increased endogenous H O levels indicate oxidative stress and could be a potential marker of many diseases, including Alzheimer's, cardiovascular diseases, and diabetes. However, consuming H O incorporated food...

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Bibliographic Details
Published inFood chemistry Vol. 421; p. 136234
Main Authors Panneer Selvam, Sathish, Cho, Sungbo
Format Journal Article
LanguageEnglish
Published England 30.09.2023
Subjects
Charcoal
DNA - genetics
Humans
Hydrogen Peroxide
Phosphates
Reactive Oxygen Species
Self-assembly
HO
Salmon testes DNA
Density functional theory
Theobroma cacao
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Summary:Hydrogen peroxide (H O ) is one of the most important reactive oxygen species (ROS). Increased endogenous H O levels indicate oxidative stress and could be a potential marker of many diseases, including Alzheimer's, cardiovascular diseases, and diabetes. However, consuming H O incorporated food has adverse effects on humans and is a serious health concern. We used salmon testes DNA with bio-inspired activated carbon (AC) as an electrocatalyst for developing a novel H O sensor. The phosphate backbone of DNA contains negatively charged oxygen groups that specifically attract protons from H O reduction. We observed a linearity range of 0.01-250.0 μM in the H O reduction peak current with a detection limit of 2.5 and 45.7 nM for chronoamperometric and differential pulse voltammetric studies. High biocompatibility of the sensor was achieved by the DNA, facilitating endogenous H O detection. Moreover, this non-enzymatic sensor could also help in the rapid screening of H O -contaminated foods.
ISSN:1873-7072