Electrochemical peptide nucleic acid functionalized α-Fe 2 O 3 /Fe 3 O 4 nanosheets for detection of CYP2C192 gene

• Published in: Mikrochimica acta (1966) Vol. 190; no. 5; p. 189
• Main Authors: Ni, Yun, Chen, Xiu, Ling, Chen, Zhu, Ziye, Yue, Yao, Wang, Jie, He, Aolin, Liu, Ruijiang
• Format: Journal Article
• Language: English
• Published: Austria 21.04.2023
• Subjects: Clopidogrel; Cytochrome P-450 CYP2C19; DNA; Peptide Nucleic Acids; Reproducibility of Results; CYP2C192; Nanosheet; α-Fe2O3/Fe3O4; Electrochemical sensor; Differential pulse voltammetry
• ISSN: 1436-5073

The CYP2C19*2 gene carriers and non-carriers are closely related to the dosage of clopidogrel. To correctly guide the use of clopidogrel and promote individualized therapy, an ultra-sensitive electrochemical biosensor was developed for the detection of CYP2C19*2 gene. The heterogeneous α-Fe O /Fe O nanosheets were prepared via the hydrothermal-calcination process, and the preparation parameters were optimized. The average diameter and thickness of the nanosheets were approximately 150 nm and 53 nm, respectively; and the saturation magnetization was 80.2 emu/g. The α-Fe O /Fe O @Au nanosheets were prepared by sodium borohydride reduction method, and self-assembled to the electrode surface with magnetic field. Ultra-sensitive detection of CYP2C19*2 gene was realized through the recognition ability of strong single base mismatching of peptide nucleic acid and signal amplification effect of magnetic α-Fe O /Fe O @Au nanosheets. Under optimal detection conditions, the current had a good linear correlation with the negative logarithm of CYP2C19*2 gene concentration in the range 1 pM-1 nM, and the detection limit was 0.64 pM (S/N = 3). Meanwhile, the electrochemical signals of target DNA and incomplete complementary DNA were detected. The constructed biosensor exhibited good selectivity, reproducibility, and stability, providing a promising strategy for the detection of other gene mutations by electrochemical biosensors.