The voltage-gated sodium channel Na V 1.7 underlies endometriosis-associated chronic pelvic pain

• Published in: Journal of neurochemistry
• Main Authors: Castro, Joel, Maddern, Jessica, Chow, Chun Yuen, Tran, Poanna, Vetter, Irina, King, Glenn F, Brierley, Stuart M
• Format: Journal Article
• Language: English
• Published: England 24.02.2023
• Subjects: chronic pelvic pain; mechanosensitivity; vagina-innervating afferents; endometriosis; visceromotor response; voltage-gated sodium channels
• ISSN: 1471-4159

Chronic pelvic pain (CPP) is the primary symptom of endometriosis patients, but adequate treatments are lacking. Modulation of ion channels expressed by sensory nerves innervating the viscera has shown promise for the treatment of irritable bowel syndrome and overactive bladder. However, similar approaches for endometriosis-associated CPP remain underdeveloped. Here, we examined the role of the voltage-gated sodium (Na ) channel Na 1.7 in (i) the sensitivity of vagina-innervating sensory afferents and investigated whether (ii) Na 1.7 inhibition reduces nociceptive signals from the vagina and (iii) ameliorates endometriosis-associated CPP. The mechanical responsiveness of vagina-innervating sensory afferents was assessed with ex vivo single-unit recording preparations. Pain evoked by vaginal distension (VD) was quantified by the visceromotor response (VMR) in vivo. In control mice, pharmacological activation of Na 1.7 with OD1 sensitised vagina-innervating pelvic afferents to mechanical stimuli. Using a syngeneic mouse model of endometriosis, we established that endometriosis sensitised vagina-innervating pelvic afferents to mechanical stimuli. The highly selective Na 1.7 inhibitor Tsp1a revealed that this afferent hypersensitivity occurred in a Na 1.7-dependent manner. Moreover, in vivo intra-vaginal treatment with Tsp1a reduced the exaggerated VMRs to VD which is characteristic of mice with endometriosis. Conversely, Tsp1a did not alter ex vivo afferent mechanosensitivity nor in vivo VMRs to VD in Sham control mice. Collectively, these findings suggest that Na 1.7 plays a crucial role in endometriosis-induced vaginal hyperalgesia. Importantly, Na 1.7 inhibition selectively alleviated endometriosis-associated CPP without the loss of normal sensation, suggesting that selective targeting of Na 1.7 could improve the quality of life of women with endometriosis.