Evaluation of the Cytotoxic Effect of Pd 2 Spm against Prostate Cancer through Vibrational Microspectroscopies

Regarding the development of new antineoplastic agents, with a view to assess the selective antitumoral potential which aims at causing irreversible damage to cancer cells while preserving the integrity of their healthy counterparts, it is essential to evaluate the cytotoxic effects in both healthy...

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Bibliographic Details
Published inInternational journal of molecular sciences Vol. 24; no. 3
Main Authors Laginha, Raquel C, Martins, Clara B, Brandão, Ana L C, Marques, Joana, Marques, M Paula M, Batista de Carvalho, Luís A E, Santos, Inês P, Batista de Carvalho, Ana L M
Format Journal Article
LanguageEnglish
Published Switzerland 18.01.2023
Subjects
Antineoplastic Agents - pharmacology
Cisplatin - pharmacology
Humans
Lipids
Male
Prostatic Neoplasms - drug therapy
Spectroscopy, Fourier Transform Infrared
Spermine - metabolism
Spermine - pharmacology
Raman microspectroscopy
prostate cancer
FTIR microspectroscopy
palladium(II)
cisplatin
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Summary:Regarding the development of new antineoplastic agents, with a view to assess the selective antitumoral potential which aims at causing irreversible damage to cancer cells while preserving the integrity of their healthy counterparts, it is essential to evaluate the cytotoxic effects in both healthy and malignant human cell lines. In this study, a complex with two Pd(II) centers linked by the biogenic polyamine spermine (Pd Spm) was tested on healthy (PNT-2) and cancer (LNCaP and PC-3) prostate human cell lines, using cisplatin as a reference. To understand the mechanisms of action of both cisplatin and Pd Spm at a molecular level, Fourier Transform Infrared (FTIR) and Raman microspectroscopies were used. Principal component analysis was applied to the vibrational data, revealing the major metabolic changes caused by each drug, which were found to rely on DNA, lipids, and proteins, acting as biomarkers of drug impact. The main changes were observed between the B-DNA native conformation and either Z-DNA or A-DNA, with a higher effect on lipids having been detected in the presence of cisplatin as compared to Pd Spm. In turn, the Pd-agent showed a more significant impact on proteins.
ISSN:1422-0067