Reactive chemiluminescence of human lymphocytes during phytohemagglutinin stimulation

The luminol-mediated chemiluminescence of highly purified human peripheral blood lymphocytes has been studied. The incubation of lymphocytes with phytohemagglutinin has lead to dose-dependent chemiluminescence, most pronounced by the end of the first minute. The kinetics of the reaction does not dep...

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Bibliographic Details
Published inŽurnal mikrobiologii, ėpidemiologii i immunobiologii no. 5; p. 64
Main Authors Rassanov, S P, Maianskiĭ, A N, Chebotar', I V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1987
Subjects
Cell Separation - methods
Dose-Response Relationship, Drug
Humans
Kinetics
Luminescent Measurements
Luminol - pharmacology
Lymphocyte Activation - drug effects
Lymphocytes - drug effects
Monocytes - drug effects
Phytohemagglutinins - pharmacology
Stimulation, Chemical
Time Factors
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Summary:The luminol-mediated chemiluminescence of highly purified human peripheral blood lymphocytes has been studied. The incubation of lymphocytes with phytohemagglutinin has lead to dose-dependent chemiluminescence, most pronounced by the end of the first minute. The kinetics of the reaction does not depend on the dose of the stimulant, the concentration of lymphocytes or the presence of mononuclear phagocytes. Monocytes (adhering cells) are not stimulated with phytohemagglutinin though their presence in the total fraction of mononuclear blood cells enhances the chemiluminescence of lymphocytes.
ISSN:0372-9311