Physical and natural cross-linking approaches on 3D gelatin microspheres for cartilage regeneration

• Published in: Tissue engineering. Part C, Methods
• Main Authors: Sulaiman, Shamsul Bin, Abdul Rani, Rizal Bin, Mohamad Yahaya, Nor Hamdan Bin, Tabata, Yasuhiko, Hiraoka, Yosuke, Seet, Wan Tai, Ng, Min Hwei
• Format: Journal Article
• Language: English
• Published: United States 26.05.2022
• ISSN: 1937-3392

The use of gelatin microspheres (GM) as a cell carrier has been extensively researched. One of its limitation is that it dissolves rapidly in aqueous settings, precluding its use for long-term cell propagation. This circumstance necessitates the use of cross-linking agents to circumvent the constraint. Thus, the current study examines two different methods of cross-linking and their effect on the microsphere's '"physicochemical and cartilage tissue regeneration capacity. Crosslinking was accomplished by physical [Dehydrothermal (DHT)] and natural (Genipin) cross-linking of the 3D gelatin microspheres (GM). We begin by comparing the microstructures of the scaffolds and their long-term resistance to degradation under physiological conditions (in isotonic solution, at 37 °C, pH = 7.4). Infrared spectroscopy indicated that the gelatin structure was preserved after the cross-linking treatments. The cross-linked GM" 'demonstrated good cell adhesion, viability, proliferation, and widespread 3D scaffold colonization when seeded with human bone marrow mesenchymal stem cells (BMSCs). Additionally, the cross-linked microspheres enhanced chondrogenesis, as demonstrated by the data. It was discovered that cross-linked GM increased the expression of cartilage-related genes and the biosynthesis of a glycosaminoglycan-positive matrix as compared to non-crosslinked GM. In comparison, DHT-crosslinked results were significantly enhanced. To summarize, DHT treatment was found to be a superior approach for cross-linking the GM in order to promote better cartilage tissue regeneration.