A new method for isolating gas vesicles from Microcystis for ultrasound contrast

Gas vesicles are a unique class of gas-filled protein nanostructures which are commonly found in cyanobacteria and . The gas vesicles may scatter sound waves and generate harmonic signals, which enabled them to have the potential to become a novel ultrasound contrast agent. However, the current hype...

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Bibliographic Details
Published inSheng wu gong cheng xue bao = Chinese journal of biotechnology Vol. 38; no. 4; p. 1589
Main Authors Xu, Rui, Long, Huan, Wang, Yinghui, Huang, Kaiyao
Format Journal Article
LanguageChinese
Published China 25.04.2022
Subjects
Contrast Media
Cyanobacteria
Hydrogen Peroxide
Microcystis
Proteins - chemistry
ultrasound contrast agent
gas vesicles
hypertonic cracking method
H2O2 method
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Summary:Gas vesicles are a unique class of gas-filled protein nanostructures which are commonly found in cyanobacteria and . The gas vesicles may scatter sound waves and generate harmonic signals, which enabled them to have the potential to become a novel ultrasound contrast agent. However, the current hypertonic cracking method for isolating gas vesicles contains tedious operational procedures and is of low yield, thus not suitable for large-scale application. To overcome these technical challenges, we developed a rapid and efficient method for isolating gas vesicles from The new H O -based method increased the yield by three times and shortened the operation time from 24 hours to 7 hours. The H O method is not only suitable for isolation of gas vesicles from laboratory-cultured , but also suitable for colonial covered with gelatinous sheath The gas vesicles isolated by H O method showed good performance in ultrasound contrast imaging. In conclusion, this new method shows great potential for large-scale application
ISSN:1872-2075