6-Benzylaminopurine Alleviates the Impact of Cu 2+ Toxicity on Photosynthetic Performance of Ricinus communis L. Seedlings

• Published in: International journal of molecular sciences Vol. 22; no. 24
• Main Authors: Sameena, Puthukkolli P, Kalaji, Hazem M, Żuk-Gołaszewska, Krystyna, Horaczek, Tomasz, Sierka, Edyta, Puthur, Jos T
• Format: Journal Article
• Language: English
• Published: Switzerland 12.12.2021
• Subjects: Benzyl Compounds - pharmacology; Copper - pharmacology; Photosynthesis - drug effects; Purines - pharmacology; Ricinus - metabolism; Seedlings - metabolism; kinetin; 6-benzylaminopurine; chlorophyll a fluorescence; cytokinin; membrane stability; reactive oxygen species; antioxidation
• ISSN: 1422-0067

Copper (Cu) is an essential element involved in various metabolic processes in plants, but at concentrations above the threshold level, it becomes a potential stress factor. The effects of two different cytokinins, kinetin (KIN) and 6-benzylaminopurine (BAP), on chlorophyll fluorescence parameters, stomatal responses and antioxidation mechanisms in castor ( L.) under Cu toxicity was investigated. plants were exposed to 80 and 160 μM CuSO added to the growth medium. Foliar spraying of 15 μM KIN and BAP was carried out on these seedlings. The application of these cytokinins enhanced the tissue water status, chlorophyll contents, stomatal opening and photosynthetic efficiency in the castor plants subjected to Cu stress. The fluorescence parameters, such as Fm, Fv/Fo, Sm, photochemical and non-photochemical quantum yields, energy absorbed, energy trapped and electron transport per cross-sections, were more efficiently modulated by BAP application than KIN under Cu toxicity. There was also effective alleviation of reactive oxygen species by enzymatic and non-enzymatic antioxidation systems, reducing the membrane lipid peroxidation, which brought about a relative enhancement in the membrane stability index. Of the various treatments, 80 µM CuSO + BAP recorded the highest increase in photosynthetic efficiency compared to other cytokinin treatments. Therefore, it can be concluded that BAP could effectively alleviate the detrimental effects of Cu toxicity in cotyledonary leaves of by effectively modulating stomatal responses and antioxidation mechanisms, thereby enhancing the photosynthetic apparatus' functioning.