The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1 H NMR

Recurrent miscarriage applies to pregnancy loss expulsion of the fetus within the first 24 weeks of pregnancy. This study is aimed at comparatively investigating the sera of women with RM with those who have no record of miscarriages to identify if there were any metabolite and metabolic pathway dif...

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Bibliographic Details
Published inBioMed research international Vol. 2021; p. 3422138
Main Authors Latifimehr, Mahbobeh, Rastegari, Ali Asghar, Zamani, Zahra, Esfahani, Pezhman Fard, Nazari, Leila
Format Journal Article
LanguageEnglish
Published United States 2021
Subjects
Abortion, Habitual - blood
Abortion, Habitual - metabolism
Adult
Discriminant Analysis
Female
Humans
Iran - epidemiology
Least-Squares Analysis
Magnetic Resonance Spectroscopy - methods
Metabolic Networks and Pathways - physiology
Metabolome
Metabolomics - methods
Pregnancy - metabolism
Proton Magnetic Resonance Spectroscopy
Serum - chemistry
Serum - metabolism
Iran
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Summary:Recurrent miscarriage applies to pregnancy loss expulsion of the fetus within the first 24 weeks of pregnancy. This study is aimed at comparatively investigating the sera of women with RM with those who have no record of miscarriages to identify if there were any metabolite and metabolic pathway differences using H NMR spectroscopy. Serum samples were collected from women with RM ( = 30) and those who had no records of RM ( = 30) to obtain metabolomics information. H NMR spectroscopy was carried out on the samples using Carr Purcell Meiboom Gill spin echo; also, Partial Least Squares Discriminant Analysis was performed in MATLAB software using the ProMetab program to obtain the classifying chemical shifts; the metabolites were identified by using the Human Metabolome Database (HMDB) in both the experimental and control groups. The pathway analysis option of the website was used to identify the changed metabolic pathways. The results of the study revealed that 14 metabolites were different in the patients with RM. Moreover, the pathway analysis showed that taurine and hypotaurine metabolism along with phenylalanine, tyrosine, and tryptophan biosynthesis was significantly different in patients with RM. The present study proposes that any alteration in the above metabolic pathways might lead to metabolic dysfunctions which may result in a higher probability of RM.
ISSN:2314-6141