Comparative study of the binding of human blood plasma fibronectin with clinical strains of staphylococci

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 12; p. 28
• Main Authors: Buchwald, F, Tabachnik, A L, Ermolin, G A, Zhidkova, N I, Kotelianskiĭ, V E
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.12.1987
• Subjects: Fibronectins - blood; Humans; Iodine Radioisotopes; Protein Binding; Staphylococcus - growth & development; Staphylococcus - metabolism; Staphylococcus aureus - growth & development; Staphylococcus aureus - metabolism; Staphylococcus epidermidis - growth & development; Staphylococcus epidermidis - metabolism
• ISSN: 0372-9311

The interaction of 62 S. aureus clinical strains and, respectively, 20 and 17 isolated S. epidermidis and S. saprophyticus strains with human blood plasma fibronectin (FN) has been studied. The specific interaction of FN with bacteria has been evaluated simultaneously by the binding of 125I with FN (method 1), the FN-mediated agglutination of staphylococci (method 2) and the character of colonies formed in 0.15% agar medium containing FN (method 3). The data obtained in this investigation indicated that all S. aureus strains under study react with FN to a different extent. When evaluating the binding of FN with bacteria, the most pronounced correlation was observed between methods 1 and 3. None of the methods used in this investigation has revealed interaction between FN and S. epidermidis and S. saprophyticus strains under study. The authors suggest that a preliminary inference on the capacity of the isolated clinical strains of staphylococci for reaction with FN may be made from the character of colonies formed in 0.15% agar medium containing FN.