Ratiometric sensing of butyrylcholinesterase activity based on the MnO 2 nanosheet-modulated fluorescence of sulfur quantum dots and o-phenylenediamine

Butyrylcholinesterase (BChE) can modulate the expression level of cholinesterase, which emerges as an important clinical diagnose index. However, the currently reported assays for BChE are suffering from the problem of interferences. A ratiometric fluorescence assay was developed based on the MnO na...

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Published inMikrochimica acta (1966) Vol. 188; no. 9; p. 294
Main Authors Ma, Zerui, Li, Pan, Jiao, Meng, Shi, Yu-E, Zhai, Yongqing, Wang, Zhenguang
Format Journal Article
LanguageEnglish
Published Austria 07.08.2021
Subjects
Butyrylcholinesterase - chemistry
Fluorescence
Humans
Manganese Compounds - chemistry
Phenylenediamines - chemistry
Quantum Dots - chemistry
Quantum dots
o-Phenylenediamine
Fluorescence
Ratiometric assay
Butyrylcholinesterase
MnO2 nanosheets
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Summary:Butyrylcholinesterase (BChE) can modulate the expression level of cholinesterase, which emerges as an important clinical diagnose index. However, the currently reported assays for BChE are suffering from the problem of interferences. A ratiometric fluorescence assay was developed based on the MnO nanosheet (NS)-modulated fluorescence of sulfur quantum dots (S-dots) and o-phenylenediamine (OPD). MnO NS can not only quench the fluorescence of blue emissive S-dots, but also enhance the yellow emissive OPD by catalyzing its oxidation reactions. Upon introducing BChE and substrate into the system, their hydrolysate can reduce MnO into Mn , leading to the fluorescence recovery of S-dots and failure of OPD oxidation. BChE activity can be quantitatively detected by recording the change of fluorescence signals in the blue and yellow regions. A linear relationship is observed between the ratio of F /F and the concentration of BChE in the range 30 to 500 U/L, and a limit of detection of 17.8 U/L has been calculated. The ratiometric fluorescence assay shows an excellent selectivity to acetylcholinesterase and tolerance to various other species. The method developed  provides good detection performances in human serum medium and for screening of  inhibitors.
ISSN:1436-5073