ISSR-Based Genetic Diversity Assessment of Genus J asminum L. (Oleaceae) from Pakistan

The genus L., of the family Oleaceae, includes many species occurring in the wild, or cultivated worldwide. A preliminary investigation based on inter-simple sequence repeats (ISSR) was performed to assess the genetic diversity among 28 accessions, representing nine species of from various regions,...

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Bibliographic Details
Published inPlants (Basel) Vol. 10; no. 7
Main Authors Akhtar, Naeem, Hafiz, Ishfaq Ahmad, Hayat, Muhammad Qasim, Potter, Daniel, Abbasi, Nadeem Akhtar, Habib, Umer, Hussain, Adil, Hafeez, Hina, Bashir, Muhammad Ajmal, Malik, Saad Imran
Format Journal Article
LanguageEnglish
Published Switzerland 22.06.2021
Subjects
genetic diversity
Jasminum L
Pakistan
polymorphism
ISSR
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Summary:The genus L., of the family Oleaceae, includes many species occurring in the wild, or cultivated worldwide. A preliminary investigation based on inter-simple sequence repeats (ISSR) was performed to assess the genetic diversity among 28 accessions, representing nine species of from various regions, representing a range of altitudes in Pakistan. A total of 21 ISSR primers were used, which produced 570 amplified bands of different sizes, with a mean polymorphic band percentage of 98.26%. The maximum resolving power, polymorphism information content, and index values of the ISSR markers recorded for primers 6, 16, and 19 were 0.40, 12.32, and 24.21, respectively. Based on the data of the ISSR markers, the resulting UPGMA dendrogram with the Jaccard coefficient divided the 28 accessions into two main clades. At the species level, the highest values for Shannon's information index, polymorphism percentage, effective allele number, Nei's genetic variations, and genetic unbiased diversity were found in L. and L., while the lowest were observed in Hance and Skan. Based on Nei's unbiased genetic identity pairwise population matrix, the maximum identity (0.804) was observed between Willd and (Burm. f.) Andrews, and the lowest (0.566) between Skan. and L. Molecular variance analysis displayed a genetic variation of 79% among the nine populations. The study was aimed to established genetic diversity in species using ISSR markers. With the help of this technique, we were able to establish immense intra- and interspecific diversity across the species.
ISSN:2223-7747
2223-7747