Changes in subcellular structures and states of Pumilio1 regulate the translation of target Mad2 and Cyclin B1 mRNAs

Temporal and spatial control of mRNA translation has emerged as a major mechanism for promoting diverse biological processes. However, the molecular nature of temporal and spatial control of translation remains unclear. In oocytes, many mRNAs are deposited as a translationally repressed form and are...

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Published inJournal of cell science
Main Authors Takei, Natsumi, Takada, Yuki, Kawamura, Shohei, Sato, Keisuke, Saitoh, Atsushi, Bormann, Jenny, Yuen, Wai Shan, Carroll, John, Kotani, Tomoya
Format Journal Article
LanguageEnglish
Published England 01.01.2020
Subjects
mRNA localization
Vertebrate
Meiosis
Translational control
Oocyte
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Summary:Temporal and spatial control of mRNA translation has emerged as a major mechanism for promoting diverse biological processes. However, the molecular nature of temporal and spatial control of translation remains unclear. In oocytes, many mRNAs are deposited as a translationally repressed form and are translated at appropriate timings to promote the progression of meiosis and development. Here, we show that changes in subcellular structures and states of the RNA-binding protein Pumilio1 regulate the translation of target mRNAs and progression of oocyte maturation. Pumilio1 was shown to bind to Mad2 and Cyclin B1 mRNAs, assemble highly clustered aggregates, and surround Mad2 and Cyclin B1 RNA granules in mouse oocytes. These Pumilio1 aggregates were dissolved prior to the translational activation of target mRNAs possibly by phosphorylation. Stabilization of Pumilio1 aggregates prevented the translational activation of target mRNAs and progression of oocyte maturation. Together, our results provide an aggregation-dissolution model for the temporal and spatial control of translation.
ISSN:1477-9137