Effect of lycopene on As 2 O 3 induced oxidative stress in SH-SY5Y cells

It is known that oxidative stress may cause neuronal injury and several experimental models showed that As O exposure causes oxidative stress. Lycopene, a carotenoid, has been shown to have protective effect in neurological disease models due to antioxidant activity, but its effect on As O -induced...

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Published inMolecular biology reports
Main Authors Oguz, Elif, Terzioglu Bebitoglu, Berna, Acet, Gokce, Hodzic, Ajla, Hatiboglu, Nebile, Ada, Saniye
Format Journal Article
LanguageEnglish
Published Netherlands 04.05.2021
Subjects
Oxidative stress
Neurotoxicity
SH-SY5Y cells
As2O3
Lycopene
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Summary:It is known that oxidative stress may cause neuronal injury and several experimental models showed that As O exposure causes oxidative stress. Lycopene, a carotenoid, has been shown to have protective effect in neurological disease models due to antioxidant activity, but its effect on As O -induced neurotoxicity is not identified yet. The aim of this study is to investigate the effects of lycopene on As O -induced neuronal damage and the related mechanisms. Cell viability was determined by the MTT assay. Lycopene was administrated with different concentrations (2, 4, 6 and 8 µM) one hour before 2 µM As O exposure in SH-SY5Y human neuroblastoma cells. The anti-oxidant effect of lycopene was determined by measuring superoxide dismutase (SOD), catalase (CAT) hydrogen peroxide (H O ), malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS). MTT results and LDH cytotoxicity analyses showed that pretreatment with 8 µM lycopene significantly improved the toxicity due to As O exposure in SH‑SY5Y neuroblastoma cells. Pretreatment with lycopene significantly increased the activities of anti‑oxidative enzymes as well as total antioxidant status and decreased total oxidative status in As O exposed cells. The results of this study indicate that lycopene may be a potent neuroprotective against oxidative stress and could be used to prevent neuronal injury or death in several neurological diseases.
ISSN:1573-4978