Effect of lycopene on As 2 O 3 induced oxidative stress in SH-SY5Y cells
It is known that oxidative stress may cause neuronal injury and several experimental models showed that As O exposure causes oxidative stress. Lycopene, a carotenoid, has been shown to have protective effect in neurological disease models due to antioxidant activity, but its effect on As O -induced...
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Published in | Molecular biology reports |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
04.05.2021
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Subjects | |
Online Access | Get full text |
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Summary: | It is known that oxidative stress may cause neuronal injury and several experimental models showed that As
O
exposure causes oxidative stress. Lycopene, a carotenoid, has been shown to have protective effect in neurological disease models due to antioxidant activity, but its effect on As
O
-induced neurotoxicity is not identified yet. The aim of this study is to investigate the effects of lycopene on As
O
-induced neuronal damage and the related mechanisms. Cell viability was determined by the MTT assay. Lycopene was administrated with different concentrations (2, 4, 6 and 8 µM) one hour before 2 µM As
O
exposure in SH-SY5Y human neuroblastoma cells. The anti-oxidant effect of lycopene was determined by measuring superoxide dismutase (SOD), catalase (CAT) hydrogen peroxide (H
O
), malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS). MTT results and LDH cytotoxicity analyses showed that pretreatment with 8 µM lycopene significantly improved the toxicity due to As
O
exposure in SH‑SY5Y neuroblastoma cells. Pretreatment with lycopene significantly increased the activities of anti‑oxidative enzymes as well as total antioxidant status and decreased total oxidative status in As
O
exposed cells. The results of this study indicate that lycopene may be a potent neuroprotective against oxidative stress and could be used to prevent neuronal injury or death in several neurological diseases. |
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ISSN: | 1573-4978 |