Progress and perspective on development of non-model industrial bacteria as chassis cells for biochemical production in the synthetic biology era

• Published in: Sheng wu gong cheng xue bao = Chinese journal of biotechnology Vol. 37; no. 3; p. 874
• Main Authors: Yang, Yongfu, Geng, Binan, Song, Haoyue, Hu, Mimi, He, Qiaoning, Chen, Shouwen, Bai, Fengwu, Yang, Shihui
• Format: Journal Article
• Language: Chinese
• Published: China 25.03.2021
• Subjects: Bacteria - genetics; Metabolic Engineering; Synthetic Biology; synthetic biology; chassis cells; non-model microorganisms; microbial cell factories; biosafety; industrial microorganism
• ISSN: 1872-2075

The development and implement of microbial chassis cells can provide excellent cell factories for diverse industrial applications, which help achieve the goal of environmental protection and sustainable bioeconomy. The synthetic biology strategy of Design-Build-Test-Learn (DBTL) plays a crucial role on rational and/or semi-rational construction or modification of chassis cells to achieve the goals of "Building to Understand" and "Building for Applications". In this review, we briefly comment on the technical development of the DBTL cycle and the research progress of a few model microorganisms. We mainly focuse on non-model bacterial cell factories with potential industrial applications, which possess unique physiological and biochemical characteristics, capabilities of utilizing one-carbon compounds or of producing platform compounds efficiently. We also propose strategies for the efficient and effective construction and application of synthetic microbial cell factories securely in the synthetic biology era,