Novel monoclonal antibody-sandwich immunochromatographic assay based on Fe 3 O 4 /Au nanoparticles for rapid detection of fish allergen parvalbumin

In this study, a rapid sandwich immunochromatographic assay (ICA) was developed to detect parvalbumin (PV). Firstly, two optimum primary monoclonal antibody (mAb) against PV had been screened out: mAb1 was used as the capture antibody, and mAb2 conjugated to Fe O /Au nanoparticles (Fe O /AuNPs) that...

Full description

Saved in:
Bibliographic Details
Published inFood research international Vol. 142; p. 110102
Main Authors Zhang, Mengke, Li, Mengyin, Zhao, Yan, Xu, Naifeng, Peng, Lanlan, Wang, Yuanfeng, Wei, Xinlin
Format Journal Article
LanguageEnglish
Published Canada 01.04.2021
Subjects
Qualitative and quantitative determination
Sandwich immunochromatographic assay
Monoclonal antibody
Parvalbumin
FeO/Au nanoparticles
Online AccessGet full text

Cover

More Information
Summary:In this study, a rapid sandwich immunochromatographic assay (ICA) was developed to detect parvalbumin (PV). Firstly, two optimum primary monoclonal antibody (mAb) against PV had been screened out: mAb1 was used as the capture antibody, and mAb2 conjugated to Fe O /Au nanoparticles (Fe O /AuNPs) that served as a detection reagent. Using this pair of mAbs, a sandwich ICA strip based on Fe O /AuNPs was developed. The results showed that the color intensity of test line positively correlated with the PV concentration in the standard or spiked sample. The limit of detection for qualitative (LOD) and quantitative detection (LOQ) were 2 ng/mL and 0.691 ng/mL, respectively. Besides, the detection time of this ICA strip was within 15 min. The recovery rates ranged from 104.0% to 117.4%, within an acceptable level (80-120%). Moreover, the developed assay also showed high cross reaction in different fish species. These results demonstrated that the established test strip has the potential to be used as a rapid screening tool for large scale determination of PV in foodstuffs.
ISSN:1873-7145