Physarum polycephalum macroplasmodium exhibits countermeasures against TiO 2 nanoparticle toxicity: A physiological, biochemical, transcriptional, and metabolic perspective

Concerns about the environmental and human health implications of TiO nanoparticles (nTiO ) are growing with their increased use in consumer and industrial products. Investigations of the underlying molecular mechanisms of nTiO tolerance in organisms will assist in countering nTiO toxicity. In this...

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Bibliographic Details
Published inEnvironmental pollution (1987) Vol. 279; p. 116936
Main Authors Zhang, Zhi, Liang, Zhi Cheng, Liang, Xiu Yi, Zhang, Qing Hai, Wang, Ya Jie, Zhang, Jian Hua, De Liu, Shi
Format Journal Article
LanguageEnglish
Published England 15.06.2021
Subjects
Animals
Humans
Metabolome
Nanoparticles - toxicity
Physarum polycephalum - genetics
Titanium - toxicity
Metabolomics
Physarum polycephalum
Transcriptomics
TiO nanoparticles
Metabolic intervention
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Summary:Concerns about the environmental and human health implications of TiO nanoparticles (nTiO ) are growing with their increased use in consumer and industrial products. Investigations of the underlying molecular mechanisms of nTiO tolerance in organisms will assist in countering nTiO toxicity. In this study, the countermeasures exhibited by the slime mold Physarum polycephalum macroplasmodium against nTiO toxicity were investigated from a physiological, transcriptional, and metabolic perspective. The results suggested that the countermeasures against nTiO exposure include gene-associated metabolic rearrangements in cellular pathways involved in amino acid, carbohydrate, and nucleic acid metabolism. Gene-associated nonmetabolic rearrangements involve processes such as DNA repair, DNA replication, and the cell cycle, and occur mainly when macroplasmodia are exposed to inhibitory doses of nTiO . Interestingly, the growth of macroplasmodia and mammal cells was significantly restored by supplementation with a combination of responsive metabolites identified by metabolome analysis. Taken together, we report a novel model organism for the study of nTiO tolerance and provide insights into countermeasures taken by macroplasmodia in response to nTiO toxicity. Furthermore, we also present an approach to mitigate the effects of nTiO toxicity in cells by metabolic intervention.
ISSN:1873-6424