Fluorescent sensor based on triphenylamine for Zn 2+ with high selectivity and imaging in living cells

It is of great importance to design a fluorescent sensor with high selectivity, sensitivity and large Stokes shift to zinc detection for environmental water sample and in vivo. Herein, A novel Zn fluorescent sensor with larger Stokes shift (110 nm) 1-((5-(4-(diphenylamino)phenyl)pyridine-2-imino)met...

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Bibliographic Details
Published inSpectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 251; p. 119480
Main Authors Wang, Dongyuan, Yin, Qiang, Zheng, Ming, Xie, Yongbo, He, Wen, Li, Zheng, Hou, Sanying, Wang, Hongqing
Format Journal Article
LanguageEnglish
Published England 15.04.2021
Subjects
Fluorescent Dyes
Ions
Spectrometry, Fluorescence
Spectroscopy, Fourier Transform Infrared
Zinc
Fluorescent sensor
Cell imaging
pH effect
Zinc
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Summary:It is of great importance to design a fluorescent sensor with high selectivity, sensitivity and large Stokes shift to zinc detection for environmental water sample and in vivo. Herein, A novel Zn fluorescent sensor with larger Stokes shift (110 nm) 1-((5-(4-(diphenylamino)phenyl)pyridine-2-imino)methyl)naphthalene-2-ol (abbr. TPA-PN) was designed and synthesized. In DMF-H O (V: V = 1: 1, pH = 7.0) solution, it could achieve high selectivity and sensitivity to Zn , there was a linear responsive range of 0-20 μM of concentration of Zn ions for the sensor, the detection limit was as low as 19.134 nM and the binding constant was calculated to be 3.24 × 10 M . The species of TPA-PN and zinc were clarified at different pH. Besides, the interaction properties and fluorescence mechanism were demonstrated by the species theory, density functional theory (DFT) calculation, H NMR titration, FT-IR and MS. Most importantly, it provided a new real-time, on-site method and showed excellent potential in-vivo imaging ability.
ISSN:1873-3557