1 H, 13 C and 15 N chemical shift assignment of the stem-loop 5a from the 5'-UTR of SARS-CoV-2

The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major p...

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Published inBiomolecular NMR assignments Vol. 15; no. 1; p. 203
Main Authors Schnieders, Robbin, Peter, Stephen A, Banijamali, Elnaz, Riad, Magdalena, Altincekic, Nadide, Bains, Jasleen Kaur, Ceylan, Betül, Fürtig, Boris, Grün, J Tassilo, Hengesbach, Martin, Hohmann, Katharina F, Hymon, Daniel, Knezic, Bozana, Oxenfarth, Andreas, Petzold, Katja, Qureshi, Nusrat S, Richter, Christian, Schlagnitweit, Judith, Schlundt, Andreas, Schwalbe, Harald, Stirnal, Elke, Sudakov, Alexey, Vögele, Jennifer, Wacker, Anna, Weigand, Julia E, Wirmer-Bartoschek, Julia, Wöhnert, Jens
Format Journal Article
LanguageEnglish
Published Netherlands 01.04.2021
Subjects
5' Untranslated Regions
Carbon Isotopes
Coronavirus Papain-Like Proteases - chemistry
Genes, Viral
Hydrogen
Magnetic Resonance Spectroscopy
Nitrogen Isotopes
Protein Binding
Protein Domains
Protein Structure, Secondary
SARS-CoV-2 - chemistry
SARS-CoV-2 - genetics
SARS-CoV-2
SL5a
5′-UTR
COVID19-NMR
Solution NMR spectroscopy
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Summary:The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis-acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5'-untranslated region (5'-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5'-UUUCGU-3' hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive H, C and N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy.
ISSN:1874-270X