The Dis1/Stu2/XMAP215 Family Gene Fg Stu2 Is Involved in Vegetative Growth, Morphology, Sexual and Asexual Reproduction, Pathogenicity and DON Production of Fusarium graminearum
The conserved Dis1/Stu2/XMAP215 microtubule association proteins (MAPs) family plays an important role in microtubule dynamics, nucleation, and kinetochore-microtubule attachments. However, function of Dis1/Stu2/XMAP215 homolog in plant pathogenic fungi has not been determined. Here, we identified a...
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Published in | Frontiers in microbiology Vol. 11; p. 545015 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
2020
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Subjects | |
Online Access | Get full text |
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Summary: | The conserved Dis1/Stu2/XMAP215 microtubule association proteins (MAPs) family plays an important role in microtubule dynamics, nucleation, and kinetochore-microtubule attachments. However, function of Dis1/Stu2/XMAP215 homolog in plant pathogenic fungi has not been determined. Here, we identified and investigated the Dis1/Stu2/XMAP215 homolog (FGSG_10528) in
(
Stu2p). Co-localization experiment and co-immunoprecipitation (Co-IP) assay demonstrated that
Stu2p is a microtubule associated protein. Besides,
Stu2 could also interact with
γ-tubulin and presumed
Ndc80, which suggested that the
Stu2 gene might associate with microtubule nucleation and kinetochore-microtubule attachments like Dis1/Stu2/XMAP215 homologs in other species. Moreover, the
Stu2 promoter replacement mutants (
Stu2-Si mutants) produced twisted hyphae and decreased growth rate. Microscope examination further showed that the microtubule polymerization was reduced in
Stu2-Si mutants, which could account for the aberrant morphology. Although the microtubule polymerization was affected in
Stu2-Si mutants, the
Stu2-Si mutants didn't show highly increased sensitivity to anti-microtubule fungicide carbendazim (methyl benzimidazol-2-ylcarbamate [MBC]). In addition, the
Stu2-Si mutants exhibited curved conidia, decreased number of conidial production, blocked ability of perithecia production, decreased pathogenicity and deoxynivalenol (DON) production. Taken together, these results indicate that the
Stu2 gene plays a crucial role in vegetative growth, morphology, sexual reproduction, asexual reproduction, virulence and deoxynivalenol (DON) production of
, which brings new insights into the functions of Dis1/Stu2/XMAP215 homolog in plant pathogenic fungi. |
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ISSN: | 1664-302X 1664-302X |