A novel near-infrared fluorimetric method for point-of-care monitoring of Fe 2+ and its application in bioimaging

Iron is one of the essential trace elements in the human body, which is involved in many important physiological processes of life. The abnormal amount of iron in the body will bring many diseases. Therefore, a novel near-infrared fluorimetric method was developed. The method is based on a fluoresce...

Full description

Saved in:
Bibliographic Details
Published inJournal of hazardous materials Vol. 406; p. 124767
Main Authors Zhu, Meiqing, Zhao, Zongyuan, Liu, Xina, Chen, Panpan, Fan, Fugang, Wu, Xiangwei, Hua, Rimao, Wang, Yi
Format Journal Article
LanguageEnglish
Published Netherlands 15.03.2021
Subjects
Fe(2+)
DFT
Imaging
Near-infrared fluorescent probe
Extremely large stokes shift
Online AccessGet full text

Cover

More Information
Summary:Iron is one of the essential trace elements in the human body, which is involved in many important physiological processes of life. The abnormal amount of iron in the body will bring many diseases. Therefore, a novel near-infrared fluorimetric method was developed. The method is based on a fluorescent probe (E)-4-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1-en-1-yl)vinyl)-N, N-diethylaniline oxide (DDED) which uses N-oxide as a recognition group to real-time monitoring and imaging of Fe in vivo and in vitro. The method exhibits excellent selectivity and high sensitivity (LOD = 27 nM) for Fe , fast reaction rate (< 4 min), extremely large Stokes shift (＞ 275 nm), low cytotoxicity. The strip test strongly illustrates the potential application of DDED in real environment. In particular, DDED has been successfully applied to real-time monitoring and imaging of Fe in HepG cells and zebrafish. That is, the method has great potential for the detection of Fe in living systems.
ISSN:1873-3336