Mitochondrial Ca 2+ Signaling Is an Electrometabolic Switch to Fuel Phagosome Killing
Phagocytes reallocate metabolic resources to kill engulfed pathogens, but the intracellular signals that rapidly switch the immunometabolic program necessary to fuel microbial killing are not understood. We report that macrophages use a fast two-step Ca relay to meet the bioenergetic demands of phag...
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Published in | Cell reports (Cambridge) Vol. 33; no. 8; p. 108411 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
24.11.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Phagocytes reallocate metabolic resources to kill engulfed pathogens, but the intracellular signals that rapidly switch the immunometabolic program necessary to fuel microbial killing are not understood. We report that macrophages use a fast two-step Ca
relay to meet the bioenergetic demands of phagosomal killing. Upon detection of a fungal pathogen, macrophages rapidly elevate cytosolic Ca
(phase 1), and by concurrently activating the mitochondrial Ca
(mCa
) uniporter (MCU), they trigger a rapid influx of Ca
into the mitochondria (phase 2). mCa
signaling reprograms mitochondrial metabolism, at least in part, through the activation of pyruvate dehydrogenase (PDH). Deprived of mCa
signaling, Mcu
macrophages are deficient in phagosomal reactive oxygen species (ROS) production and defective at killing fungi. Mice lacking MCU in their myeloid cells are highly susceptible to disseminated candidiasis. In essence, this study reveals an elegant design principle that MCU-dependent Ca
signaling is an electrometabolic switch to fuel phagosome killing. |
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ISSN: | 2211-1247 |