Whole-cell biosynthesis of 2-O-α-D-glu-copyranosyl-sn-glycerol by recombinant Bacillus subtilis

2-O-α-D-glu-copyranosyl-sn-glycerol is a high value-added product with prospective application in food, cosmetics, health products and pharmaceutical industries. However, industrial scale of 2-O-α-D-glu-copyranosyl-sn-glycerol has not yet been applied in China, and there are few related reports on 2...

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Published inSheng wu gong cheng xue bao = Chinese journal of biotechnology Vol. 36; no. 9; p. 1918
Main Authors Duan, Peifeng, You, Jiajia, Xu, Meijuan, Yang, Taowei, Shao, Minglong, Zhang, Xian, Rao, Zhiming
Format Journal Article
LanguageChinese
Published China 25.09.2020
Subjects
Bacillus subtilis - genetics
China
Glycerol
Prospective Studies
Sucrose
China
2-O-α-D-glu-copyranosyl-sn-glycerol
Bacillus subtilis
whole cell catalysis
heterologous expression
sucrose phosphorylase
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Summary:2-O-α-D-glu-copyranosyl-sn-glycerol is a high value-added product with prospective application in food, cosmetics, health products and pharmaceutical industries. However, industrial scale of 2-O-α-D-glu-copyranosyl-sn-glycerol has not yet been applied in China, and there are few related reports on 2-O-α-D-glu-copyranosyl-sn-glycerol synthesis. The purpose of this experiment is to develop a method for catalyzing the synthesis of food-grade 2-O-α-D-glu-copyranosyl-sn-glycerol using whole cells of "Generally Recognized as Safe" (GRAS) recombinant Bacillus subtilis. In our work, a recombinant B. subtilis 168/pMA5-gtfA that heterologously expressing Leuconostoc mesenteroides sucrose phosphorylase was constructed and used as a whole-cell catalyst to synthesize 2-O-α-D-glu-copyranosyl-sn-glycerol. Optimizing the culture temperature, time and whole cell transformation conditions has increased the yield of 2-O-α-D-glu-copyranosyl-sn-glycerol. The results showed that 1.43 U/mL of sucrose phosphorylase was achieved in B
ISSN:1872-2075