Mass spectrometry-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from follicular fluid

Couples with infertility issues have been assisted by in vitro fertilization reproduction technologies with high success rates of 50-80%. However, complications associated with ovarian stimulation remain, such as ovarian hyperstimulation. Oocyte quality is a significant factor impacting the outcome...

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Bibliographic Details
Published inBiomolecular concepts Vol. 11; no. 1; p. 153
Main Authors Klobučar, Marko, Pavlić, Sanja Dević, Car, Iris, Severinski, Neda Smiljan, Milaković, Tamara Tramišak, Badovinac, Anđelka Radojčić, Pavelić, Sandra Kraljević
Format Journal Article
LanguageEnglish
Published Germany 25.10.2020
Subjects
Adult
Female
Fertilization in Vitro
Follicular Fluid - metabolism
Glycomics - methods
Humans
Immunoglobulin G - analysis
Mass Spectrometry
Ovulation Induction
Proteomics - methods
In vitro fertilization
MALDI mass spectrometry
inflammation
glycoproteins
follicular fluid glycome
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Summary:Couples with infertility issues have been assisted by in vitro fertilization reproduction technologies with high success rates of 50-80%. However, complications associated with ovarian stimulation remain, such as ovarian hyperstimulation. Oocyte quality is a significant factor impacting the outcome of in vitro fertilization procedures, but other processes are also critical for fertilization success. Increasing evidence points to aberrant inflammation as one of these critical processes reflected in molecular changes, including glycosylation of proteins. Here we report results from a MALDI-TOF-MS-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from the follicular fluid obtained from patients undergoing fertilization through either (1) assisted reproduction by modified natural cycle or (2) controlled ovarian stimulation (GnRH antagonist, GnRH Ant) protocols. Significant inflammatory-related differences between analyzed N-glycomes were observed from samples and correlated with the ovarian stimulation protocol used in patients.
ISSN:1868-503X