Bisbenzylisoquinoline alkaloids of Cissampelos sympodialis with antiviral activity against dengue virus

A number of bisbenzyilisoquinoline alkaloids have been previously isolated from (Menispermaceae). The tertiary alkaloid fraction of the rhizomes (TAFrz) was prepared and the major alkaloid warifteine was isolated. Five TAFrz subfractions in addition to warifteine were tested against Dengue virus (DE...

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Published inNatural product research p. 1
Main Authors da Silva, Poliana G, Fonseca, Aventino H, Ribeiro, Malu P, Silva, Taizia D, Grael, Cristiane F F, Pena, Lindomar J, Silva, Tania M S, Oliveira, Eduardo de J
Format Journal Article
LanguageEnglish
Published England 29.09.2020
Subjects
Cissampelos sympodialis
methylwarifteine
dengue virus
antiviral activity
bisbenzylisoquinoline alkaloids
warifteine
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Summary:A number of bisbenzyilisoquinoline alkaloids have been previously isolated from (Menispermaceae). The tertiary alkaloid fraction of the rhizomes (TAFrz) was prepared and the major alkaloid warifteine was isolated. Five TAFrz subfractions in addition to warifteine were tested against Dengue virus (DENV). We then used an epithelial (Vero) cell line to evaluate the cytotoxicity and effective concentrations of the samples against DENV. All TAFrz subfractions were active, but subfraction 6 (a mixture of the alkaloids methylwarifteine and warifteine) in particular showed a promising antiviral effect against DENV-2 with an IC of 2.00 µg/mL and a selectivity index (SI) of 10.74. Warifteine was the second most active sample and had an IC of 8.13 µg/mL and SI = 10.94. The antiviral activity of the samples compared favorably with that of 6-methylmercaptopurine riboside (IC = 7.31 µg/mL and SI = 11.8). These results suggest that bisbenzylisoquinoline alkaloids may prove interesting leading antiviral compounds.
ISSN:1478-6427