Fatigue-induced Fos immunoreactivity within the lumbar cord and amygdala decreases after С 60 fullerene pretreatment

The fundamental aspects related to the mechanisms of action of C fullerene nanoparticles on the level of the central nervous system in different experimental conditions are still unclear. Electrophysiological investigation and immunohistochemical techniques of c-fos expression were combined to deter...

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Published inScientific reports Vol. 10; no. 1; p. 9826
Main Authors Maznychenko, Andriy V, Bulgakova, Nataliya V, Sokolowska, Inna V, Butowska, Kamila, Borowik, Agnieszka, Mankivska, Olena P, Piosik, Jacek, Tomiak, Tomasz, Gonchar, Olga O, Maisky, Volodymyr O, Kostyukov, Alexander I
Format Journal Article
LanguageEnglish
Published England 17.06.2020
Subjects
Amygdala - drug effects
Amygdala - metabolism
Amygdala - physiology
Animals
Antioxidants - metabolism
Electrophysiological Phenomena - drug effects
Fullerenes - chemistry
Fullerenes - pharmacology
Male
Muscle Fatigue - drug effects
Proto-Oncogene Proteins c-fos - metabolism
Rats
Rats, Wistar
Spinal Cord - drug effects
Spinal Cord - metabolism
Spinal Cord - physiology
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Summary:The fundamental aspects related to the mechanisms of action of C fullerene nanoparticles on the level of the central nervous system in different experimental conditions are still unclear. Electrophysiological investigation and immunohistochemical techniques of c-fos expression were combined to determine which neural elements within the lumbar segments and in the central nucleus of the amygdala (CeA) are activated under skeletal muscle fatigue development with prior application of C fullerenes (dissolved in dimethyl sulfoxide and in distilled water, FDS). After high-frequency electrical stimulation of the triceps surae muscle, the main fatigue-related increases in the c-Fos expression level were registered ipsilaterally within lamina 1 and 5 of the lumbar segments and within the contralateral capsular part of the CeA. C fullerene pretreatment in animals with subsequent electrical stimulation induced a distinct (2-4 times) decrease in the level of Fos immunoreactivity in the observed structures in comparison with only fatigue-induced rats. It can be supposed that FDS, as antioxidant compound, can decrease the concentration of free radicals in fatigued tissue and reduce the transmission intensity of nociceptive information from muscles to the spinal cord and amygdala, thereby changing the level of c-Fos expression within the lumbar segments and CeA.
ISSN:2045-2322