Porous graphene based electrochemical immunosensor using Cu 3 (BTC) 2 metal-organic framework as nonenzymatic label
An electrochemical immunosensor for highly sensitive detection of cancer biomarkers has been developed based on the combination of a sensing platform of polydopamine modified porous graphene and a nonenzymatic label of metal-organic framework (MOF) conjugated secondary antibody. This approach achiev...
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Published in | Talanta (Oxford) Vol. 217; p. 121042 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
01.09.2020
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Subjects | |
Online Access | Get full text |
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Summary: | An electrochemical immunosensor for highly sensitive detection of cancer biomarkers has been developed based on the combination of a sensing platform of polydopamine modified porous graphene and a nonenzymatic label of metal-organic framework (MOF) conjugated secondary antibody. This approach achieves a wide range of linear response from 0.1 to 10 ng/mL, low detection limit of 0.025 ng/mL (at a signal to noise ratio of 3), good reproducibility and selectivity for the detection of prostate specific antigen (PSA) as a model analyte. The high performance of the immunosensor is attributed to the high surface area from porous graphene and the strong adhesion of polydopamine, allowing a high load of the primary antibody of PSA, as well as the highly electrocatalytic activity of the Cu
(BTC)
(BTC = benzene-1,3,5-tricarboxylic acid) MOF toward H
O
to provide greatly amplified sensitivity. In this respect, the MOF-based nonenzymatic label shows promising application for the point-of-care detection of different cancer biomarkers in clinical diagnostics. |
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ISSN: | 1873-3573 |