Nitrate removal characteristics and 13 C metabolic pathways of aerobic denitrifying bacterium Paracoccus denitrificans Z195

Strain Z195 was isolated and identified as Paracoccus denitrificans. Z195 exhibited efficient aerobic denitrification and carbon removal abilities, and removed 93.74% of total nitrogen (TN) and 97.81% of total organic carbon.71.88% of nitrogen was lost as gaseous products. C-metabolic flux analysis...

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Bibliographic Details
Published inBioresource technology Vol. 307; p. 123230
Main Authors Zhang, Haihan, Li, Sulin, Ma, Ben, Huang, Tinglin, Qiu, Hui, Zhao, Zhenfang, Huang, Xin, Liu, Kaiwen
Format Journal Article
LanguageEnglish
Published England 01.07.2020
Subjects
Aerobiosis
Bacteria
Denitrification
Metabolic Networks and Pathways
Nitrates
Nitrification
Nitrogen
Paracoccus denitrificans
Nitrate removal
Stable isotopic labeling
Paracoccus denitrificans
Aerobic denitrifying
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Summary:Strain Z195 was isolated and identified as Paracoccus denitrificans. Z195 exhibited efficient aerobic denitrification and carbon removal abilities, and removed 93.74% of total nitrogen (TN) and 97.81% of total organic carbon.71.88% of nitrogen was lost as gaseous products. C-metabolic flux analysis revealed that 95% and 132% of the carbon fluxes entered the Entner-Doudoroff (ED) pathway and tricarboxylic acid (TCA) cycle, respectively. Electrons produced by carbon metabolism markedly promoted the processes of nitrogen metabolism process and aerobic respiration. A response surface methodology model demonstrated that the optimal conditions for the maximum TN removal were a C/N ratio of 7.47, shaking speed of 108 rpm, temperature of 31 °C and initial pH of 8.02. Additionally, the average TN and chemical oxygen demand removal efficiencies of raw wastewater were 89% and 91%, respectively. The results give new insight for understanding metabolic flux analysis of aerobic denitrifying bacteria.
ISSN:1873-2976