Rapid Differentiation of Asian and American Ginseng by Differential Ion Mobility Spectrometry-Tandem Mass Spectrometry Using Stepwise Modulation of Gas Modifier Concentration
This study reports a rapid and robust method for the differentiation of Asian and American ginseng samples based on differential ion mobility spectrometry-tandem mass spectrometry (DMS-MS/MS). Groups of bioactive ginsenoside/pseudo-ginsenoside isomers, including Rf/Rg /F , Rb /Rb /Rc, and Rd/Re, in...
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Published in | Journal of the American Society for Mass Spectrometry Vol. 30; no. 11; p. 2212 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.11.2019
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Subjects | |
Online Access | Get full text |
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Summary: | This study reports a rapid and robust method for the differentiation of Asian and American ginseng samples based on differential ion mobility spectrometry-tandem mass spectrometry (DMS-MS/MS). Groups of bioactive ginsenoside/pseudo-ginsenoside isomers, including Rf/Rg
/F
, Rb
/Rb
/Rc, and Rd/Re, in the ginseng extracts were sequentially separated using DMS with stepwise changes in the gas modifier concentration prior to MS analysis. The identities of the spatially separated ginsenoside/pseudo-ginsenoside isomers were confirmed by their characteristic compensation voltages at specific modifier loading and MS/MS product ions. As expected, Asian ginseng samples contained some Rf and an insignificant amount of F
, whereas American ginseng samples had a high level of F
but no Rf. The origin of the whole and sliced ginseng could further be confirmed using the quantitative ratios of three sets of ginsenoside markers, namely, Rg
/Re, Rb
/Rg
, and Rb
/Rc. Based on our results, new benchmark ratios of Rg
/Re < 0.15, Rb
/Rg
> 2.15, and Rb
/Rc < 0.26 were proposed for American ginseng (as opposed to Asian ginseng). |
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ISSN: | 1879-1123 |