Transcriptome-wide map of m 6 A circRNAs identified in a rat model of hypoxia mediated pulmonary hypertension

• Published in: BMC genomics Vol. 21; no. 1; p. 39
• Main Authors: Su, Hua, Wang, Guowen, Wu, Lingfang, Ma, Xiuqing, Ying, Kejing, Zhang, Ruifeng
• Format: Journal Article
• Language: English
• Published: England 13.01.2020
• Subjects: m6A circTmtc3; m6A circXpo6; Hypoxia mediated pulmonary hypertension; m6A circRNAs

Hypoxia mediated pulmonary hypertension (HPH) is a lethal disease and lacks effective therapy. CircRNAs play significant roles in physiological process. Recently, circRNAs are found to be m A-modified. The abundance of circRNAs was influenced by m A. Furthermore, the significance of m A circRNAs has not been elucidated in HPH yet. Here we aim to investigate the transcriptome-wide map of m A circRNAs in HPH. Differentially expressed m A abundance was detected in lungs of HPH rats. M A abundance in circRNAs was significantly reduced in hypoxia in vitro. M A circRNAs were mainly from protein-coding genes spanned single exons in control and HPH groups. Moreover, m A influenced the circRNA-miRNA-mRNA co-expression network in hypoxia. M A circXpo6 and m A circTmtc3 were firstly identified to be downregulated in HPH. Our study firstly identified the transcriptome-wide map of m A circRNAs in HPH. M A can influence circRNA-miRNA-mRNA network. Furthermore, we firstly identified two HPH-associated m A circRNAs: circXpo6 and circTmtc3. However, the clinical significance of m A circRNAs for HPH should be further validated.